Postdoctoral Researcher at the Department of Bioengineering, Imperial College London

Dr Bettina Bohl

I’m interested in developing and engineering advanced model systems for the human brain based on induced pluripotent stem cells (iPSCs). By monitoring neuronal function on a cellular, molecular and neurochemical level, I’m aiming to contribute to a more complete picture of human neurophysiology and neurological diseases.

Asymmetric Cell Division

DNA

Vesicles

Receptors

CV

  • Oct 22 - present Postdoctoral Research Assistant: Dep. of Bioengineering, Imperial College London

    Nov 21 - Jun 22 Postdoctoral Research Assistant: Ins. for Biochemistry, Friedrich-Alexander-University Erlangen

    Feb 16 - Sep 21 PhD project: Hector Institute for Translational Brain Research, University of Heidelberg/Medical Faculty Mannheim

  • Jan 15 - Jan 16 Master Project: Ins. of Cell Biology, Friedrich-Schiller-University Jena

    Sep - Dec 14 ERASMUS+ funded Internship: Dep. of Neuroscience and Pharmacology, University of Copenhagen

    Mar 14 Internship: Dep. of Biochemistry, Friedrich-Schiller-University Jena

    Oct - Dec 13 Research Assistant: Fritz-Lipmann-Institute Jena

    Apr - Sep 13 Internship and Bachelor Project: Dep. of Biochemistry, University Hospital Jena

  • Oct 13 - Jan 16 M.Sc. Biochemistry, Friedrich-Schiller-University Jena

    Oct 10 - Sep 13 B.Sc. Biochemistry and Molecular Biology, Friedrich-Schiller-University Jena

Technical expertise

  • Induced Pluripotent Stem Cell (iPSC) Techniques

    Differentiation of iPSC-derived Neurons in 2D and 3D Brain Organoids

    Differentiation of iPSC-derived Oligodendrocytes & Microglia

    Genome Editing via CRISPR/Cas9

    Transduction with Lentiviruses and Adeno-associated viruses

    Cultivation of Human Cell Lines and Mammalian Primary Cells

  • Molecular Cloning (incl. Gibson Assembly, Site-directed Mutagenesis)

    DNA/RNA Isolation

    PCRs (genotyping, RT-PCR, qPCR)

    Preparation of cDNA-libraries for RNA Sequencing

  • Immunofluorescence Staining

    SDS-PAGE, Western Blot

    Co-Immunoprecipitation

    Gradient Centrifugation

  • Widefield Fluorescence Microscopy

    Confocal Microscopy

    Live-Cell Imaging (Calcium Imaging, Cell Tracking)

  • Fast-Scan Cyclic Voltammetry

    Fast-Scan Controlled-Adsorption Voltammetry

  • 3D Printing

    Photolithography

    PDMS Chip Fabrication

    Carbon Electrode Fabrication

  • Image Processing (ImageJ, CellProfiler, Imaris)

    Statistical Data Analysis (GraphPad Prism, R)

    Design (AutoCAD)

Publications

Cam M, Durieu E, Bodin M, Manousopoulou A, Koslowski S, Vasylieva N, Barnych B, Hammock BD, Bohl B, Koch P et al.: Induction of Amyloid-β42 Production by Fipronil and Other Pyrazole Insecticides. Journal of Alzheimer's disease JAD 2018;62(4):1663–81.

Bohl B, Jabali A, Ladewig J, Koch P. Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells. Science Advances. 2022 Feb 11;8(6):eabl5792.

Jabali A, Hoffrichter A, Uzquiano A, Marsoner F, Wilkens R, Siekmann M, Bohl B, Rossetti AC, Horschitz S, Koch P, Francis F, Ladewig J. Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation. EMBO Reports. 2022 May 4;23(5):e54027.

Wilkens R, Hoffrichter A, Kleinsimlinghaus K, Bohl B, Haag C, Lehmann N, Schmidt M, Muñoz Perez-Vico E, Wangemann J, Rehder KF, Horschitz S, Köhr G, Ladewig J, Koch P. Diverse maturity-dependent and complementary anti-apoptotic brakes safeguard human iPSC-derived neurons from cell death. Cell Death & Disease. 2022 Oct 21;13(10):887.

Kremp M, Aberle T, Sock E, Bohl B, Hillgärtner S, Winkler J, Wegner M. Transcription factor Olig2 is a major downstream effector of histone demethylase Phf8 during oligodendroglial development. Glia. 2024 Aug;72(8):1435-1450.

News

  • ITTS Meeting - Bethesda

    Nov 2024

    Thanks to Adithya Gopinath and Gisela Andrea Camacho-Hernandez for organising an amazing Young Scientist Symposia at the ITTS meeting in Washington. I really enjoyed my first invited talk, and the amazing feedback on our Symposium.

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  • MMiN Meeting - Chapel Hill

    May 2024

    Current and former Hashemi lab members met at the MMiN meeting in North Carolina. After three inspiring days and presenting my poster, I felt greatly honoured to be elected one of the scientific advisors for the upcoming MMiN meetings. Looking forward to meet all the lovely people back in Amsterdam in 2026.

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  • Imperial Lates

    Feb 2023

    Your brain on drugs…and how electrochemistry can be used to monitor brain chemistry and drug impact. The whole Hashemi lab was presenting our work at Imperial Lates and enjoying a vibrant evening with exciting scientific discussions.

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  • New City, New Challenges

    Oct 2022

    I’m so excited and grateful to join the Hashemi lab at Imperial College as a postdoctoral researcher. Looking forward to dive into the field of neurochemistry, and exploring all the opportunities at the Dep of Bioengineering.

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  • Farbe im Quadrat

    Nov 2021

    Together with colleagues, we participated in the “Farbe im Quadrat”-initiative to embellish the city centre of Mannheim. We redesigned a junction box in the neighbourhood aiming to make our research approachable to the local community.

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Funding